Some observations on the crystalline lens

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                                34

<u>Solutions of lens protein</u>. were prepared.

Ox lenses were dried and crushed and shaken
with distilled water. The mixture was
centrifuged and the [text?] solution poured
off and filtered. This solution  became opaque
after 15 minutes exposure to the mercury-vapour
lamp.

The solution was dialysed for two days
against frequent changes of distilled water.
On standing, a precipitate of globulin settled.
The remaining globulin was precipitated by
half saturation with ammonium sulfate and
the solution dialysed against distilled water for
a further two days. The solution of lens
albumen thus prepared was exposed to
the radiations from the mercury-vapour
lamp, the temperature being kept below 20°C.
It became slightly opalescent in about half
an hour, the opalescence increasing at the
                            
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Manuscript details

Author
Clare Harris
Reference
MS/165
Series
MS
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Some observations on the crystalline lens , From The Royal Society, MS/165

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